K31117

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K31117

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)
  • low saturation (PATO:0000328)
    (Seed::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K31117

Determination of introduced cDNA(s)

  • J033008E09 - Rice Full-length cDNAs - Original: J033008E09

    full reading [+] show sequence

    Nucleotide sequence: J033008E09 - full reading 
    GCCCGCATCA CCCCCACCTC GTCAGGTTTC CGTTCCGCCT CCGCCTCTGC 
    TGCTCCTCCT CCTCCTCGCC GCCGCCGCCG CCGCGACGAG GCATGGAGCT 
    CGCGCGATCG GCCTGCTCCA AGGAGCACCA CAAGATCTAC GCCGGCTGGT 
    TCGCCGTCGC CAACCCAGAT GGAGATGGGC GTGTCACGGG TGCTGATGCT 
    ACCAAGTTCT TCGCCATGTC CGGCCTGTCC CGCGCCGACC TCAAGCAGGT 
    CTGGGCGATC GCGGACACCA AGCGGCAGGG GTTCCTCGGC TTCGGCGAGT 
    TCGTCGCCGC GATGCAGCTC GTGTCACTGG CGCAAGCGGG GGAGGAGATC 
    ACTCAGGACA GTCTGAAGCG CGAAGACCTG AGTAGTTTGG ATCCACCTGT 
    GATGGAAGGT CTTGACGAAC TACTTGCGAG ATCAAAGGCT GGGGTAAAGA 
    GAGTACACCC AGAAGAAAAT GGCGCCCCTC AGGTTCAAGT GCATTCTGCT 
    AATAGCTGGT TCAGTTCAAA ATCAGCAAAG AAGATGCAGG TCCCCCTGAC 
    TGCTGTTACT TCTGTAATTG ATGGCTTAAA AAGACTATAC GTAGAAAAAC 
    TGAAGCCTCT GGAGGTTGCA TACCGGTTCA ACGATTTTGC TTCCCCGTTA 
    TTGACAAGCA GTGATTTTGA TGCAAAACCA ATGGTTATGC TCTTGGGTCA 
    ATATTCTACA GGGAAAACAA CTTTCATTAA GCATCTGCTG CAAACAAGTT 
    ATCCAGGAGC TCATGTTGGA CCAGAGCCCA CTACTGACCG ATTTGTTGTT 
    GTCATGTCTG GATCTGATGA AAGGACTATT CCTGGCAATA CTATTGCTGT 
    TCAAGCTGAT ATGCCATTCA CTGGTCTTAC GACATTTGGC GGTGCTTTTC 
    TGTCAAAGTT CGAGTGCTCT CAGATGCCAC ATCCGCTGCT CGATCATATC 
    ACCTTTGTGG ACACCCCCGG TGTCCTGTCT GGTGAAAAGC AGCGGACGCA 
    GCGTAGCTAT GATTTCGCCG GCATAACTTC ATGGTTTGCT GCTAAGTGTG 
    ACCTTATTCT TCTTCTGTTT GATCCTCATA AGCTTGATAT CAGTGATGAG 
    TTCAAACGTG TGATTGCATC TTTACGTGGC CATGATGACA AAATACGTGT 
    AGTGCTGAAT AAGGCAGACC AAGTTGACAC TCAGCAGCTT ATGAGAGTGT 
    ATGGTGCACT GATGTGGTCT CTTGGGAAAG TACTCAATAC CCCTGAGGTC 
    GCGCGTGTTT ATATCGGGTC ATTCAATGAC AAACCAGTGA ATGAATCAGC 
    TGTTGGACCA ATTGGAAAGG ACTTGTTTGA GAAAGAGCAA GATGATCTCC 
    TCTGTGATCT GAAAGACATA CCCAAGAAGG CCTGTGATCG TCGGGTCAAC 
    GAGTTTGTTA AACGTGCTAG AGCTGCTAAG ATTCATGCTT ACATAATTGG 
    CCATCTAAAG AAGGAGATGC CTGCAATGAT GGGGAAAGCT AAGGCTCAAC 
    AGCGACTCAT AGACAACCTG GGTGATGAGT TTGCAAAGGT CCAAAGGGAG 
    TACCATCTTC CAGCCGGAGA CTTCCCTGAC GTTGAGCACT TCAAGGAGGT 
    GTTGGGTGGG TACAACATCG ACAGGTTCGA GAAGATGAAG CCCAAGCTGG 
    TGCAGGCCGT GGACGACATG CTCGCGTACG ACATCCCGGA GCTCCTCAAG 
    AACTTCAGGA ACCCTTACGA GTGAGCCCGC TGACCTCTCG TCAGTTGTAA 
    GATAAAGATG ACCCCGCTCG GTCGTGAGTC GTGACACTGT ATGTGAGTGA 
    CACCTGATGA TGAAGGAATA TGTTGTCAAG GCGAGACATG GACGGTTTTC 
    TACTTTACCT GCCCATCTTT TGC

    Gene models with high sequence identity

    1. AT3G20290.2 [+] show detail - EPS15 homology domain 1
      E-value: 0; Score: 870.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther EH DOMAIN CONTAINING/PAST 1, 2, 3 1.1e-164
    HMMPanther EH DOMAIN 1.1e-164
    HMMPfam Dynamin, GTPase domain 1.3e-19
    superfamily P-loop containing nucleoside triphosphate hyd 4.3e-22
    superfamily EF-hand 9.2e-21
    Coil coiled-coil 0
    HMMSmart EPS15 homology (EH) 5.3e-13
    Gene3D EF-hand-like domain 2.1e-19
    Gene3D no description 6.4e-08
    ProfileScan EPS15 homology (EH) 12.579
    ProfileScan EF-HAND 2 10.385

    See the detailed result >

    AT3G20290.2

    Model type
    Protein coding
    Short Description
    EPS15 homology domain 1
    Curator Summary
    Encodes AtEHD1, one of the Arabidopsis Eps15 homology domain proteins involved in endocytosis (AtEHD2, At4g05520).
    Computational Description
    EPS15 homology domain 1 (EHD1); FUNCTIONS IN: GTP binding, GTPase activity, calcium ion binding; INVOLVED IN: endocytosis; LOCATED IN: endosome, microsome, membrane, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: EF-HAND 2 (InterPro:IPR018249), EPS15 homology (EH) (InterPro:IPR000261), Dynamin, GTPase domain (InterPro:IPR001401), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048); BEST Arabidopsis thaliana protein match is: EPS15 homology domain 2 (TAIR:AT4G05520.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR