K27436

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K27436

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K27436

Determination of introduced cDNA(s)

  • J033068O06 - Rice Full-length cDNAs - Original: J033068O06

    full reading [+] show sequence

    Nucleotide sequence: J033068O06 - full reading 
    GATAATATTT TGCTGGAGAA TAGTCGGATG CAGGAGGCGA AAAAAGATTC 
    GATCTTTGGA GGCCTTGCCT CTGGGGCGCC CGTCGGAGCG GATCGCTTCC 
    CGTTTGATCC TCGGGAGTAT TCGCCGGCGA TGAGCTCCGA CTCCAGGGAG 
    CTGCTCGGGA TCGATCCCCC GGAGCTCATC TTCCCCTTCG AATTAAAGAA 
    GCAAATCTCG TGTTCTCTGC ACCTAACAAA CAAGACAGAT GAATATGTCA 
    CGTTTAAGGT TAAGACGACT AGTCCAAAGA AGTACTGCGT CCGTCCAAAT 
    AACGGCATTG TGGCTCCCCA GTCCACATCC AATGTTCTTG TAACAATGCA 
    AGCTCAGCGG GAGGCGCCAC CGGACATGCA ATGCAAGGAC AAATTCCTTG 
    TGCAGAGTGC CATAGTGACA CAAGAGCTCA CACCGAAGGA TATCACCGGA 
    GACATGTTTA CGAAAGAATC AGGTAATGTG GTGGATGAGG TGAAGTTAAA 
    GGTTGTTTAT ACTCAACCTC ACCCGACATC TCTCAATGGC GGGTCTGAGG 
    AAGGTCTAGG AAGCTTGAGT TATCAAGAAG CAACAAAAGG ATCTAGAGAA 
    TCAGAAACAG TAACCTCTGA GCCATTGGCC TTGATTTCAA AGTTGAAGGA 
    GGAGAAGAGT TCTGCCATTC AACAGAACAT GAAGCTTCGA GAAGAACTGG 
    ATCTTCTAAG GAGGCAAATG GGGAGTCAGC ACGGTGGTTT CTCACTAGTT 
    TTTGTGCTGG TTATTGCTAT TCTGGGAATC CTGCTTGGTT TTCTCATTAA 
    GAGATGAGCG TCTGTGATCA TATATCTTGT TCCACTTTTC TGTGAAAGAG 
    TTGATAGTCG TGGGCCTTGT CTGTTGTGTT AGCCATTTGT ACCAACTCAT 
    GATCGTCTTA TCTTAGGACC TGATTCCTCT TGTAATTTCT GACTATCAAA 
    TTCCAATCAG TAGCTACTGC ATCCAGTCAA TAAGTCGTCT GTCCATTTAG 
    CATGTTTGAG TATAAGTTTT TTTTGGTCAA TTGTATTCAT CTTTTGACAC 
    CTGTAAATAT GACACTTCCC TTAACATTAT GGCTTGCGAT ATGTATATGC 
    TGGATCCTTG TG

    Gene models with high sequence identity

    1. AT2G45140.1 [+] show detail - plant VAP homolog 12
      E-value: 1e-52; Score: 204.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily PapD-like 1.8e-35
    Gene3D PapD-like 1.5e-23
    ProfileScan Major sperm protein 29.16
    HMMPanther VESICLE-ASSOCIATED MEMBRANE PROTEIN (VAMP) 3e-48
    HMMPIR Vesicle-associated membrane protein 1.1e-109
    Coil coiled-coil 0
    HMMPfam Major sperm protein 3.1e-30

    See the detailed result >

    AT2G45140.1

    Model type
    Protein coding
    Short Description
    plant VAP homolog 12
    Curator Summary
    Encodes a Plant VAMP-Associated protein that localizes to the ER and binds to the sitosterol-binding protein ORP3a. The WFDE motif in ORP3a appears to be important for this direct interaction. Mutation of this motif causes ORP3a to relocalize to the Golgi and cytosol. The interaction between PVA12 and ORP3a does not appear to be sterol-dependent.
    Computational Description
    plant VAP homolog 12 (PVA12); FUNCTIONS IN: structural molecule activity; LOCATED IN: cytosol, endoplasmic reticulum, nucleus, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: PapD-like (InterPro:IPR008962), Major sperm protein (InterPro:IPR000535), Vesicle-associated membrane protein (InterPro:IPR016763); BEST Arabidopsis thaliana protein match is: vesicle associated protein (TAIR:AT3G60600.1); Has 1106 Blast hits to 1079 proteins in 208 species: Archae - 0; Bacteria - 0; Metazoa - 441; Fungi - 140; Plants - 434; Viruses - 0; Other Eukaryotes - 91 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR