K23242

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K23242

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K23242

Determination of introduced cDNA(s)

  • J013093O11 - Rice Full-length cDNAs - Original: J013093O11

    full reading [+] show sequence

    Nucleotide sequence: J013093O11 - full reading 
    GACATCGATC AACTCCTTCC CCAAGTCGAA GCAAAATCCC TAGCGATGTC 
    GTCGCCGCCG CCCGCCGCCG CCGCCGCGAT GGCCGTCGAC GACGCCGACG 
    ACGACCAGCT CGCCTCCATG TCCACGGAGG ACATCGTCAG GGCAACCCGC 
    CTCCTCGACA ACGAGACCCG CGTCCTCAAG GATGAGCTGC AGCGGACGAA 
    CCTTGAGGTG GAATCCTACA AGGAGAAGAT CAAGGAGAAC CAGGAGAAGA 
    TCAAACTCAA CAAGCAGCTG CCTTACCTTG TCGGCAACAT CGTCGAGATA 
    TTGGAAATGA ACCCCGAGGA TGAGGCTGAA GAGGATGGTG CGAACATCGA 
    TCTGGGGATT TGGTTGGAGT CAACAAAGAC AGCTACTTGA TACTAGACAC 
    GCTACCTTCA GAATATGATT CACGGGTTAA GGCAATGGAG GTAGATGAAA 
    AGCCTACAGA GGATTACAAT GACATTGGTG GCCTTGAGAA ACAGATTCAA 
    GAGCTTGTTG AAGCAATAGT ATTGCCCATG ACTCACAAGG ACCGGTTTCA 
    GAAGTTGGGA ATTCGCCCCC CGAAAGGTGT TCTTTTGTAC GGACCACCTG 
    GCACTGGAAA GACCCTAATG GCTCGTGCAT GTGCTGCCCA AACTAACGCC 
    ACATTTCTGA AACTTGCTGG TCCACAGCTT GTCCAGATGT TCATCGGTGA 
    TGGTGCAAAG CTTGTCCGTG ATGCCTTTCA GCTTGCAAAG GAGAAATCCC 
    CCTGCATCAT TTTTATTGAT GAAATTGACG CAATTGGAAC AAAGCGTTTT 
    GATAGTGAAG TTAGTGGTGA CAGAGAAGTG CAGCGCACAA TGTTGGAGTT 
    GCTGAATCAG CTAGATGGAT TCAGTAGTGA CGAGAGGATA AAGGTGATAG 
    CTGCAACTAA TCGCGCGGAC ATTCTTGATC CTGCTTTGAT GAGGTCTGGT 
    AGGCTGGACC GAAAAATTGA GTTCCCTCAT CCATCCGAAG AAGCACGAGC 
    TAGAATTTTG CAAATCCATT CAAGAAAAAT GAATGTAAAC CCTGATGTCA 
    ACTTTGAAGA GTTGGCACGA TCTACGGACG ACTTCAATGG TGCACAACTG 
    AAGGCCGTTT GTGTAGAAGC AGGCATGCTT GCCCTACGCC GAGATGCTAC 
    AGAGGTGACA CACGAGGATT TCAATGAAGG GATCATACAA GTGCAAGCCA 
    AAAAGAAGTC CAGTTTAAAT TATTACGCTT AATTCTTCCG AGAGAGTTAT 
    TAGGCCTAAG GAAACGCTCC GTCCAAAATT GTTTGTTTGT AACACTACCG 
    TTTTATATAT TTGTGTGGTT CAAGATGTGA TTGTACGAAA CATGTCTTGC 
    GCTGTTCCTT CTCTGCTTGA TATATACTGA TAGAAGTTGA CTCGTCC

    Gene models with high sequence identity

    1. AT3G05530.1 [+] show detail - regulatory particle triple-A ATPase 5A
      E-value: 0; Score: 102.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam ATPase, AAA-type, core 1.3e-42
    superfamily P-loop containing nucleoside triphosphate hyd 2.9e-69
    PatternScan ATPase, AAA-type, conserved site 0
    Coil coiled-coil 0
    HMMSmart ATPase, AAA+ type, core 3.4e-23
    HMMPanther 26S PROTEASE REGULATORY SUBUNIT 6A 3.5e-247
    HMMPanther 26S PROTEASE REGULATORY SUBUNIT 3.5e-247
    HMMTigr 26S proteasome subunit P45 1.2e-120
    Gene3D no description 1.4e-58
    Gene3D no description 1.4e-18

    See the detailed result >

    AT3G05530.1

    Model type
    Protein coding
    Short Description
    regulatory particle triple-A ATPase 5A
    Curator Summary
    Encodes RPT5a (Regulatory Particle 5a), one of the six AAA-ATPases of the proteasome regulatory particle. Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija (Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female gametophyte lethal phenotype.
    Computational Description
    regulatory particle triple-A ATPase 5A (RPT5A); CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-type, core (InterPro:IPR003959), ATPase, AAA-type, conserved site (InterPro:IPR003960), 26S proteasome subunit P45 (InterPro:IPR005937); BEST Arabidopsis thaliana protein match is: 26S proteasome AAA-ATPase subunit RPT5B (TAIR:AT1G09100.1); Has 33265 Blast hits to 30927 proteins in 3145 species: Archae - 1437; Bacteria - 12044; Metazoa - 4947; Fungi - 3672; Plants - 3322; Viruses - 56; Other Eukaryotes - 7787 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR