K22319

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K22319

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased width (PATO:0000600)
    (Seed::Shape::wide)
  • low saturation (PATO:0000328)
    (Seed::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K22319

Determination of introduced cDNA(s)

  • J013088A04 - Rice Full-length cDNAs - Original: J013088A04

    full reading [+] show sequence

    Nucleotide sequence: J013088A04 - full reading 
    TGTTTCTGGC AGAATTGATT GGTGCAGTAT GAAGTTCTCA TCCTCACCAA 
    TATATGAAGC TAACAACAAA CATATGACAC ATTGCCATTC TTGTAAAGAT 
    ATTGATTTAC TGCAGACAAA AGATGGTCCT TTCTGCAGGT GCATCCTTAA 
    AAATTCCATT GTGTGTACCC CTCATAATAA CATCTTTTAT GTTATTAGTG 
    GGTTTCTTGA CTTAGATGCA AATTCCTGTT TGCCTCAACA TGATGGGACT 
    GCTGTTACCT ACAAAGATTA TTTTAAGACT TGGCATGGTC TTACTTTGAC 
    TTTTGAAAAT CAGCCGCTCT TGGCTGGCAG TAAACATGTG AAAGTGCGGA 
    ACTTCCTCCA CAACTGCTAC TCTAAAAAAG AAAAAGATCC TGGAAGCATT 
    AACAACAAAG AAATGCCAAG AAGAATTTTC ACAGGAATCA TTAGAAACAC 
    TTGGGGATTC TTTCCTCAAA TATGTTACAA CTCGGCACCT TTTTAGTGAA 
    TACAGGCTTC AACATGAAGG CATATTAACA AAAATGAAGA AAAACTTGAT 
    CTCCAATGCA GCTCTGTGCC AGCTGGCCTG CAGCAGTAAT CTTGTGGGTT 
    ACATTCATGC TGAGGAGTTT AATCCAAGAG ATTGGATCAT CCCTTGCTTG 
    GACTATGATG AGCGTGATAA CAAAAAAATT TCCTTTCTGG CACCCAATGG 
    TATGTACAGC CAAAGGAAAA TGTCTATTAA AAGTAAGAGG ATTGCGGATT 
    CAGTTGAGGC CTTAATTGGG GCTTACCTCA GTACTGCTGG GGAAAAGGCA 
    GCATTTCTTT TGATGAAATC ATTAGGGATG AATATAGAAT TTCACACTGA 
    AATTCCAGTT GAAAGGAAAA TTTCAATGAA AGCTGAAGAG TTCATTAATG 
    TGAGGAGCTT AGAGGGAATG CTTGGTTACA AGTTCAATGA TTCTTTATTA 
    TTGCTCGAAG CATTGACACA TGGCTCATAT CAGACTTCTG GCCCTACTTC 
    ATGCTATCAG AGGCTCGAGT TTCTTGGAGA TGCTATTTTG GATCATCTTT 
    TCACTGAATA TTACTACAGC AAATACCCTG ACTGTACTCC AGAATTGTTG 
    ACAGATCTTA GATCTGCCTC TGTGAACAAC AATTGTTACG CGCATGCAGC 
    TGTTAAATCA GGATTAAACA AGCATATCCT TCACTCATCA TCAGAACTGC 
    ATAGGAAGAT GAGCTACTAC CTTGAGGAAT TTGGGCAGTC ATTTACAGGT 
    CCATCTTATG GCTGGGAAGC GGGCATTGGT CTACCAAAGG TTCTCGGGGA 
    TGTGATTGAA TCAATAGCTG GTGCCATTTA TCTCGACAGC AAGTGTGACA 
    AGGAGGTTGT TTGGAGAAGC ATGAAGCGGC TATTGGAACC TCTTGCCACC 
    CCCGAAACCA TCGAGCCTGA CCCGGTGAAA GGGCTGCAAG AATTTTGTGA 
    TCGTAGATCA TTCAAAATAA CCTACGAAAA AAACCATGTT GATGGAGTCT 
    CATCGGTCAT TGCTCGGGTG AAGGCTGGAG AGACTACATA CTCCGCAACA 
    AAATCAGGTC CCTGCAAGTT GGTGGCGAAA AAGTTGGCAT CAAAGGCTGT 
    GCTTAAAGAT CTGATTGCTG GTCACAAAGA TACAGAGGCT GCTGCAGTAT 
    AGTGATACTT AACTATTTAA GCGTGCAACA GTAGCTAACA ATTGATATAG 
    ATAGTTGCGC ACAATAGAAA AGTTTTGGTT GGATTCATAC ATGTAATTTG 
    GAAATGCTCC TTTTTGTGTG CTCGCAAACG ATGCGTGTGA ACGGCCTCAT 
    GAATTCTGGT AGGTTTTCAT GCTTCAAACT TACAAGTATC GCAATTTTGT 
    AAATTACAAA TACGGGCATA GCACACCTGC CCATGCCATG CAGTAGTTTT 
    TTTTTTCCTC GAAATAGTAG CATTAGTTGT ATATTACTCA TAGCATCAGA 
    TTGTACAGAA AGCATGCCAG CAAAAACGAA AAAATGTACA GACAGCATTT 
    TGTTCGCATC AAGGGAAAAC TTGTTTT

    Gene models with high sequence identity

    1. AT3G03300.3 [+] show detail - dicer-like 2
      E-value: 4e-91; Score: 332.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Ribonuclease III 7.5e-25
    Gene3D Ribonuclease III 2.3e-37
    HMMSmart Ribonuclease III 2.7e-22
    HMMSmart Ribonuclease III 2.5e-30
    HMMSmart Double-stranded RNA binding 0.0069
    ProfileScan Argonaute/Dicer protein, PAZ 13.157
    ProfileScan Double-stranded RNA binding 10.12
    ProfileScan Ribonuclease III 34.508
    ProfileScan Ribonuclease III 21.476
    HMMPfam Argonaute/Dicer protein, PAZ 3.4e-11
    HMMPfam Ribonuclease III 8.8e-24
    HMMPfam Ribonuclease III 4.3e-23
    PatternScan Ribonuclease III 0
    superfamily PAZ domain 3.6e-14
    superfamily Ribonuclease III 3.2e-40
    superfamily Ribonuclease III 2.2e-26
    HMMPanther DICER-1 3e-59
    HMMPanther HELICASE-RELATED 3e-59

    See the detailed result >

    AT3G03300.3

    Model type
    Protein coding
    Short Description
    dicer-like 2
    Curator Summary
    Encodes a Dicer-like protein that functions in the antiviral silencing response in turnip-crinkle virus-infected plants but not in TMV or CMV-strain-Y-infected plants. Involved in the production of ta-siRNAs. Partially antagonizes the production of miRNAs by DCL1. Substitutes for DCL4 to produce viral siRNA when DCL4 is missing or inhibited. Able to produce siRNAs but not miRNAs.
    Computational Description
    dicer-like 2 (DCL2); FUNCTIONS IN: in 7 functions; INVOLVED IN: defense response to virus, maintenance of DNA methylation, production of ta-siRNAs involved in RNA interference; LOCATED IN: intracellular; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA/RNA helicase, DEAD/DEAH box type, N-terminal (InterPro:IPR011545), Double-stranded RNA-binding (InterPro:IPR001159), Argonaute/Dicer protein, PAZ (InterPro:IPR003100), Ribonuclease III (InterPro:IPR000999), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Dicer double-stranded RNA-binding fold (InterPro:IPR005034), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021); BEST Arabidopsis thaliana protein match is: dicer-like 4 (TAIR:AT5G20320.1).
    Link
    InterPro Scan - TAIR