K16231

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K16231

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K16231

Determination of introduced cDNA(s)

  • J013134O04 - Rice Full-length cDNAs - Original: J013134O04

    full reading [+] show sequence

    Nucleotide sequence: J013134O04 - full reading
    GGTGATGCTC TCGCACATCG ATTGGTGGAA CAGAGTATCC CGGTCAAAGC 
    CATTAGCCAC CATCCCGACG TCTTCCTCCA TCTCGCATAT CTCTGCAATG
    GCAGGGAATG CGTCAGTGAT GCGGAGGCTC TACCTCTCTT TCTACAACTG
    GATTGTCTTC ATCGGATGGG TTCAGGTGTC GTGGTCCATG ATATTGGCAT
    TGTTGGAGAA CGGATATGAG GCCGTCTATG CTGCCGTAGA GCAGCATCTG
    CTGTTCGCGC AGACTGCTGC CATCATGGAG ATTCTTCACT CCATTGTAGG
    TGAGATATCC TCTTTCTTTA TAAAGAAAAT AATAAGTAAT AAGGGTTGGT
    GAGGTCTCCA GTGTCTTCTA CTATTCCACA AATCACTGGA AGGTTGTTCA
    TGATCTGGGG CATCTTGTGG AGCTTCCCTG AGGTAATATC AGGACCTTAC
    TCCAAGTTAT AAGAAGGTTT ATATCTTAAA AAACAAGTTT TGGATTAGAA
    AGCACTTCTT AGAATGATGA AACATAGATG TTAGGTAATG TTGCAGCTAT
    TAGAACATAT CCCATACTTT CATTTATGAT CAAAATGACT CTAAGTAGGC
    ATTGGACAAA CCGAAGCTAC AGAAATTGCT TAAGTAATGA AGCGGATGCA
    ATTACATCAT GGAGAAGGAC AAAATAGTGT TCTTTTCCTA GATTCCTATT
    TCATGTATTT AACTTCTAAT CAAGTAATTG TTTTATTTAT AAACAATATC
    GGTGAATCAC TGTCCACATG TTTTGCAGAC ACACACTCAT ATTTTTGTTA
    CCTCCTTGCT CATAAGCTGG TGCATCACTG AGGTATATGT TACTTCCTAA
    AACTTCTCAC CAAATTACTC ACTTGGAAGA TCATGGATTT CGGATGCAAT
    TTTATTATAG TTCTGCTTCT TGAAATGACA GTAGTTGTTG AAGTTCCTGA
    AAGAAAATCC CAAGCTTTGA AAGTTCCTGT GTTGAGCTTA GTTGAACAAA
    TATTATGAAA TTTTCTTCAC TTCAGAGCAT GACTTTGTAG ACAAAAAATT
    ACTAATTCCT TGCAGGTTAC CAGATATTGT TTCTATGGCA TGAAGGAGTC
    ATTTGGATTT ACACCTTCCT GGCTCTTATG GCTTAGGTTG GCTCTAGTCG
    TAAACAAATC ATAATATGTA GCATAAACTT CAGCGAATTC TCTTAAAAAT
    ATGCTGCTGT TACTTCCATA CTAATTACTT TGCAGGTACA GCACCTTCAT
    AGTATGCCTT CCTGTTGGTA CGGTTAGTGA GGTTGGCCTA ATCTACATTG
    TTCTTCCTTT CATGAAGGCA TCTGGGAAAT ACTGCCTTAG GATGCCCAAC
    AAATGGAATT TCTCCTTCAA CTACTTCTAT GCCTCTATCT TTTTCATGGT
    TTTGTATGCC CCAGTATATC CACGCTTGTT TCGCTACTTG ATCGCCCAGC
    GGAAGAAGGC CTTGGCAAAA GCAAAAACCA CATAACTATA CTCAAGAGCT
    ATCTACTCTG ATTTCTGCTC GCGGCTACAG CTCAGGTTGC TACCGTTTCT
    TGAGCAAATA AGTAAAAGTG TATTAGAATG GAAATGCCGA AATACAGACT
    ATCTTTTCCC TTGGTAAATA AAAATGTACA CGAGTACCAT TTTCATGATT
    TGCTAATGGT GTGTCCTACC AAAGATCGGC TTTTCCTTCT GTAAATAAAA
    TTGTACTCCA TATCTGTGCC C

    Gene models with high sequence identity

    1. AT5G10480.3 [+] show detail - Protein-tyrosine phosphatase-like, PTPLA
      E-value: 5e-27; Score: 54.70

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther PHOSPHATASE RELATED PROTEIN PTPLA 1.1e-12
    HMMPanther Protein-tyrosine phosphatase-like, PTPLA 1.1e-12
    HMMPanther Protein-tyrosine phosphatase-like, PTPLA 2.8e-21
    HMMPfam Protein-tyrosine phosphatase-like, PTPLA 3.9e-21

    See the detailed result >

    AT5G10480.3

    Model type
    Protein coding
    Short Description
    Protein-tyrosine phosphatase-like, PTPLA
    Curator Summary
    Protein tyrosine phosphatase-like involved in cell division and differentiation. Interacts with CDKA;1 only in its phosphorylated form, preventing dephosphorylation. Overexpression slowed down cell division in suspension cell cultures at the G2-to-M transition and early mitosis and inhibited Arabidopsis seedling growth. Localized in the cytoplasm of dividing cells but moved into the nucleus upon cell differentiation. Based on complementation of yeast mutant PAS2 has acyl-CoA dehydratase activity. It interacts with CER10, a component of the microsomal fatty acid elongase complex, suggesting a role in synthesis of VLCFAs (very long chain fatty acids).
    Computational Description
    PASTICCINO 2 (PAS2); CONTAINS InterPro DOMAIN/s: Protein-tyrosine phosphatase-like, PTPLA (InterPro:IPR007482); BEST Arabidopsis thaliana protein match is: Protein-tyrosine phosphatase-like, PTPLA (TAIR:AT5G59770.1).
    Link
    InterPro Scan - TAIR