K15316

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K15316

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K15316

Determination of introduced cDNA(s)

  • J023096L07 - Rice Full-length cDNAs - Original: J023096L07

    full reading [+] show sequence

    Nucleotide sequence: J023096L07 - full reading 
    GTCACACCAA CCCGACATTC CCTCGTGCTC TTCCCATCCG CGACTCCAAA 
    ATTCCCGTCC GCTCCTCCAG GCAAGCAAGC ACGGAAACCC ACTCATCACT 
    CACTCACTCC CCCAAACCCA AACCCTAGCT ACTACTAGCT CCCCGCTCCG 
    GATCAAGCGC ATCTCGAGTT CGAGCGAGCA TTCCCCTCTT CGGTTCGAGT 
    GGGTTGGCTG GCGGCAGATG GGCTCCGACT CCAAGGAGTT GCTCGGGATC 
    GAGCCGCTCG AGCTCCGGTT CTCCTTCGAG ACGAAGAAGC AGATCTCATG 
    CTCCATGCAG CTGACGAACA GAACAGATGA CTACATCGCC TTTAAGGTCA 
    AGACAACTAG CCCAAAGAAG TACTGCGTCA GGCCGAACAG TGGCATTGTG 
    CCGCCACGGT CCACCAGCGA TGTCATCGTA ACAATGCAAG CCCAACGGGA 
    GGCACCAGCA GACATGCAGT GCAAAGACAA GTTTCTAGTG CAGAGCGTTG 
    TGGTGACTGA AGGCACAACA ACCAAGGATG TCACTGGAGA GATGTTTAAC 
    AAAGGATCAG GTAATGTGGT GGATGAGAGG AAGTTGAAGG TTGTCTATGT 
    CCAACCACCA CGACCACCAT CCCCCGTGCG TGAGGGATCT GAAGAAGGCT 
    CGTCTCCTCG GGCTTCATTA TCAGAAGGGG GCAACTTGAA TTATCAAGAT 
    ACAACTCGGG AATCTGATGA GCCACCATTG CTATCTGCTA CAAGAGCACA 
    CAAGGATGCA GAAGATGTAA CATCTGATTC TGAGACATCT GCTTTGATTT 
    CAAGATTGAC AGAAGAAAGA AACTCTGCTA TTCAGCAGAA CAATAAGCTT 
    CTCGAGGAGA TGGACCTTGT GAGGAGGGAA ATTAGCAAGC AGAATGGTGG 
    TTTCCCTTTT GTGTTCGTGG TGGTCGTTGC TCTTCTGGGA ATTCTACTGG 
    GGTATATCAT GAAGAGATAG ATGAGCTTGT CTAGTTCTGT TTCGTTAGGC 
    CTTTTCTTCC TTAAGAATTG ATTTGTTGTC TGCATTTTCT GATGCGTTTG 
    CTCACATGTT CTACACTACC GCAGTCCGCA TAGCAAATTC ATCCTAGGAT 
    CTGGTATATT CCGTAATTGC GTGCTTCAGA TTGGCTGGCT ACCATACACA 
    GTCAATAACT CATCTCCTTA ACACTAAGTT GCAAGTTAGT CGCGAGCTTG 
    GAGAGCTTAT GCAGTTAAGA AGTTTGCGCA TCGGTATCTG TTGGTACAAC 
    TGTAAACTTG CTTTCAATTT ACTAGTGTCC TGATTGCCTG ATTCTG

    Gene models with high sequence identity

    1. AT2G45140.1 [+] show detail - plant VAP homolog 12
      E-value: 1e-71; Score: 267.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Major sperm protein 2.7e-30
    Coil coiled-coil 0
    superfamily PapD-like 9.8e-36
    HMMPIR Vesicle-associated membrane protein 1.3e-91
    HMMPanther VESICLE-ASSOCIATED MEMBRANE PROTEIN (VAMP) 8.6e-57
    ProfileScan Major sperm protein 30.252
    Gene3D PapD-like 4.7e-24

    See the detailed result >

    AT2G45140.1

    Model type
    Protein coding
    Short Description
    plant VAP homolog 12
    Curator Summary
    Encodes a Plant VAMP-Associated protein that localizes to the ER and binds to the sitosterol-binding protein ORP3a. The WFDE motif in ORP3a appears to be important for this direct interaction. Mutation of this motif causes ORP3a to relocalize to the Golgi and cytosol. The interaction between PVA12 and ORP3a does not appear to be sterol-dependent.
    Computational Description
    plant VAP homolog 12 (PVA12); FUNCTIONS IN: structural molecule activity; LOCATED IN: cytosol, endoplasmic reticulum, nucleus, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: PapD-like (InterPro:IPR008962), Major sperm protein (InterPro:IPR000535), Vesicle-associated membrane protein (InterPro:IPR016763); BEST Arabidopsis thaliana protein match is: vesicle associated protein (TAIR:AT3G60600.1); Has 1106 Blast hits to 1079 proteins in 208 species: Archae - 0; Bacteria - 0; Metazoa - 441; Fungi - 140; Plants - 434; Viruses - 0; Other Eukaryotes - 91 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR