K08205

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K08205

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K08205

Determination of introduced cDNA(s)

  • J023092N19 - Rice Full-length cDNAs - Original: J023092N19

    full reading [+] show sequence

    Nucleotide sequence: J023092N19 - full reading 
    GGCCCAGTTC AAAAAAGAAC AAAAATCGCC ATTAGCTGGC TGCTAGTGGA 
    GTATTTCTGG GCGGGGCGCT CTCTTCCCTT CCCCCCGCGC CTCCACTTCC 
    GCCTCCGCCT CGCGCCACCG ACCATGCCTT CCTCCGCCGC AGCCGCCGCC 
    GACGGCGGCG ACATCGAGGA GGTGGACCGC CTCAAGAAGA GGATCAGGAC 
    CCTCGAGATG GAGGAGACGA AGTTGAACCA GCATATGGAA CGCGTCATCC 
    AGGGAATCGA AGCGAACGAG GCGTACCTCG TCGGCATGGT CCTCAGGATT 
    ATGGAAAAGG GCCCAGAGGA TGAGACTGCT GAGGATGATT GTGATGTTGG 
    CTTTCATTTG CAGCGAAAAA TTATATTCCG TCCTATTGCT GGGGTAGTGT 
    ACCCTAGCAA ACTTAAGCCT GGAGATTTAA TTGGTGTTGA CAGTACATCA 
    AATGAACACT ACTGTGGCAT TGGTGGTCTC GAAAAACAAA TTGAGGAGCT 
    TGTGGAAGCA GTTGTGTTGC CAATAATCCA CAAGAACTGC TTCCAGAGGT 
    TGGGAATTCA CCCTCCAAAA GGTGTACTTC TGTATGGACC ACCTGGAACT 
    GGGAAGACAC TCGTTGCCCA TGCATTTGCT TCCCAGACAA ATGCAACATT 
    TCTTAAGCTA ACTGGTCCAC AACTTGCCGT GAAGCTTATT GGTGAGGGAG 
    CAAGACTTGT TCGTGATGCC TTTCAGCTTG CTAAAGAGAA AGCCCCATGC 
    ATTATTTTCA TTGATGAAAT TGATGCAATT GGAAGCAAGC ACTTTGATAG 
    TGGTGATAGG GAAGTGCAAC AAACAATTGT GGAGCTTCTT AATCAGTTGG 
    ATGGCGTCGG GAGCTATGAG AGTATAAAGG TAATAGCTGC AACTAATCGG 
    CCAGAAGTTC TTGATCCTGC TTTTCTGCGA TCTGGCCGTT TAGATCAAAA 
    GATTGAATTC CCTCATCCAT CTGAACAGGC TAGAGTTAGA ATTCTGGAGA 
    TCCACTCCCG AAAAATGGAC AAAAACCCTG ATGTAAACTT TGAAGAGTTG 
    GCTTGCTGTA CAGATGATTT TAATGGTGCA CAGCTAAAGG CTGTATGCTT 
    TGAGGCTAGC ATGCTGGCAT TTCACCGAGA TGCTACAGAG GTTCGGCATG 
    AGGACTTTGT CAGAGCAATT GCGCAAGTAA AAGACGGGAA TTACTGAACA 
    ACAGCTTCAA CTACTATGCA TAGAGGACAC CATCTAACAT CTTACCTTGT 
    GTGCAGTTGG TTCTGAGACC AGACCAAGAA GCTACTGTCA CCTAACTCAA 
    ATTTTTACCA TGTCTTAGAT ACGAGAGATC AAGTATAGTT TGAAAACAGT 
    GGTCTGTATA GTACTGATTA GTAAGTTGAG AAATATTTGA GGCTCATAGT 
    AGGAAGCTAA TCAGAAAAGG CAACAATTGT TTTTGATAAC CTTGCAAGAT 
    TTGAGTTTTT TTGACAACAG CATTTGCTGA TC

    Gene models with high sequence identity

    1. AT3G05530.1 [+] show detail - regulatory particle triple-A ATPase 5A
      E-value: 8e-114; Score: 407.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart ATPase, AAA+ type, core 1.1e-21
    Coil coiled-coil 0
    superfamily P-loop containing nucleoside triphosphate hyd 1.6e-62
    Gene3D no description 1.8e-52
    Gene3D no description 4.2e-16
    PatternScan ATPase, AAA-type, conserved site 0
    HMMPanther 26S PROTEASE REGULATORY SUBUNIT 6A 3.7e-158
    HMMPanther 26S PROTEASE REGULATORY SUBUNIT 3.7e-158
    HMMPfam ATPase, AAA-type, core 1.6e-39
    HMMTigr 26S proteasome subunit P45 2e-104

    See the detailed result >

    AT3G05530.1

    Model type
    Protein coding
    Short Description
    regulatory particle triple-A ATPase 5A
    Curator Summary
    Encodes RPT5a (Regulatory Particle 5a), one of the six AAA-ATPases of the proteasome regulatory particle. Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija (Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female gametophyte lethal phenotype.
    Computational Description
    regulatory particle triple-A ATPase 5A (RPT5A); CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-type, core (InterPro:IPR003959), ATPase, AAA-type, conserved site (InterPro:IPR003960), 26S proteasome subunit P45 (InterPro:IPR005937); BEST Arabidopsis thaliana protein match is: 26S proteasome AAA-ATPase subunit RPT5B (TAIR:AT1G09100.1); Has 33265 Blast hits to 30927 proteins in 3145 species: Archae - 1437; Bacteria - 12044; Metazoa - 4947; Fungi - 3672; Plants - 3322; Viruses - 56; Other Eukaryotes - 7787 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR