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GT16949

CSHL Arabidopsis genetrap mutant lines

Original: GT16949

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT16949

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 1580275 [Map]; Strand: c
      • AT4G03560.1 - [+] show detail - two-pore channel 1
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: GT16949 - 3-prime edge 
    ACGGTAGAGG TATTTTACCG ACCGTTACCG ACCGTTTTCA TCCCTATTGG 
    TAGAGATAGT CTTGGTGGTG GTGGTACGGA TCGGGTTCGT CGATCAGAAG 
    CTATCACGCA TGGTACTCCG TTTCAGAAAG CAGCTGCACT CGTTGATCTG 
    GTTCTCTTAT TCTCTTACAC CCTAATTTTG AATTCGTTTT CCTCTTTGAC 
    TTTTCAAAAA GTTTCTATAC ATGGATCACA TCGCCTACAA GAGAGATTCT 
    ATGTATTTTA TCAGTTCATT AAGTCAGTGT TTTTGTTAAG CTGAGAAGAT 
    GAATCGTTTC TTCTTCTGAG TGATTTCTTC GTATAAGGAT TAGTCAATGC 
    TTTTGTGTGT ATATCCTCAC GAAATTAGCA TTGTTTGAAA ATTGGGAATG 
    GAGATATCTG TTGTTTCATT GTCTCTAAAG TCTTATGTCA AGAGGATTGT 
    TGGATTGACT CTGATATGTG GTATGTAGGC TGAAGATGGT ATTGGTCTTC 
    CTGTGGAAAT ACTTGATCAG TCGAGTTTCG GGGAGTCTGC TAGGTATTAC 
    TTCATCTTCA CACGTTTGGA TCTGATCTGG TCACTCAACT ATTTCGCTCT 
    GCTTTTCCTT AACTTCTTTG AGGTCAGGAC ATTTCTTTGG TATACACCAA 
    CCTTGCTTGA AAAATTTATA GAAAATCACA ACTTGAAAGT AATCACACAC 
    TTTTCCTGTC TTTTGCAGCA ACCATTGTGG TGTGAAAAAA ACCCTAAACC 
    GTCTTGCAAA GATAGAGATT ACTATTACCT GGGAGAGTTA CCGTACTTGA 
    CCAATGCAGA ATCCATTATC TATGAGGCAA GTAATTACAC TTAAG

AT4G03560.1

Model type
Protein coding
Short Description
two-pore channel 1
Curator Summary
Encodes a depolarization-activated Ca(2+) channel. Anti-sense experiments with this gene as well as Sucrose-H(+) symporters and complementation of yeast sucrose uptake mutant cch1 suggest that this protein mediates a voltage-activated Ca(2+ )influx. Mutants lack detectable SV channel activity suggesting TPC1 is essential component of the SV channel. Patch clamp analysis of loss of function mutation indicates TPC1 does not affect Ca2+ signaling in response to abiotic and biotic stress.
Computational Description
two-pore channel 1 (TPC1); FUNCTIONS IN: voltage-gated calcium channel activity, calcium channel activity; INVOLVED IN: regulation of jasmonic acid biosynthetic process, calcium ion transport, calcium-mediated signaling, seed germination, regulation of stomatal movement; LOCATED IN: vacuolar membrane, plasma membrane, vacuole, plant-type vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: EF-HAND 2 (InterPro:IPR018249), Ion transport (InterPro:IPR005821), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR