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GT15684

CSHL Arabidopsis genetrap mutant lines

Original: GT15684

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT15684

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 20728302 [Map]; Strand: c
      • AT5G50940.1 - [+] show detail - RNA-binding KH domain-containing protein
        Distance: 1823; Region: intergenic
      • AT5G50950.1 - [+] show detail - FUMARASE 2
        Distance: -1310; Region: intergenic
    Flanking Genome Sequence: GT15684 - 3-prime edge
    TATTTTACCG ACCGTTACCG ACCGTTTTCA TCCCTACTGG TTGGTCCATA 
    TCTAATGCTA TGTGCAACAA GAATTTACTC AAAGTGAATC TTGTCCCAGT
    TATTGCGAAG GTATAGAAAC AACTACCGAA TGTGTTTAAT GAGGGTCTCC
    AAGAAATGAA TCTTCCGCCT TGGAACGATT TCCTGGCAAT ATGAATATTG
    AAAGGACTTC AAATTACGAA TCTTTGGAGC AATTGCAATA GCATGATAGA
    GACAGTCCTG TGAGCTTGGA TAACCAAAGT TGAGCTGGAC AGACTCTTAA
    CTGCTCACAA GAGAGATTAG TGTTTGAAGA TTCAAAGACA CTCCCCTTAC
    GTCAAGAAAG ATTAGTGTTA GAAGATTTAC CTGG

AT5G50940.1

Model type
Protein coding
Short Description
RNA-binding KH domain-containing protein
Computational Description
RNA-binding KH domain-containing protein; FUNCTIONS IN: DNA binding; INVOLVED IN: transcription from RNA polymerase II promoter; LOCATED IN: DNA-directed RNA polymerase II, core complex; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: RNA polymerase II, heptapeptide repeat, eukaryotic (InterPro:IPR000684); BEST Arabidopsis thaliana protein match is: KH domain-containing protein (TAIR:AT4G10070.1); Has 146 Blast hits to 142 proteins in 35 species: Archae - 0; Bacteria - 0; Metazoa - 47; Fungi - 13; Plants - 78; Viruses - 0; Other Eukaryotes - 8 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G50950.1

Model type
Protein coding
Short Description
FUMARASE 2
Curator Summary
Encodes a fumarase enzyme initially shown to be in the mitochondria through proteomic studies but later shown to be present in the cytosol using an RFP fluorescent protein tag. It appears to be important for the accumulation of fumarate from malate in leaves in the light, and helps to promote nitrogen assimilation under high nitrogen conditions. It does not appear to be necessary for lipid metabolism and seedling growth.
Computational Description
FUMARASE 2 (FUM2); FUNCTIONS IN: fumarate hydratase activity, protein binding; INVOLVED IN: nitrate assimilation, response to salt stress, fumarate metabolic process; LOCATED IN: cytosol, mitochondrion; EXPRESSED IN: male gametophyte, guard cell, root, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Fumarate hydratase, class II (InterPro:IPR005677), L-Aspartase-like (InterPro:IPR008948), Lyase 1, N-terminal (InterPro:IPR022761), Fumarate lyase, conserved site (InterPro:IPR020557), Fumarase C, C-terminal (InterPro:IPR018951), Fumarate lyase (InterPro:IPR000362); BEST Arabidopsis thaliana protein match is: fumarase 1 (TAIR:AT2G47510.2); Has 20040 Blast hits to 20032 proteins in 2805 species: Archae - 403; Bacteria - 13208; Metazoa - 329; Fungi - 298; Plants - 117; Viruses - 0; Other Eukaryotes - 5685 (source: NCBI BLink).
Link
InterPro Scan - TAIR