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53-4243-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 4786983 [Map]; Strand: c
      • AT5G14800.1 - [+] show detail - pyrroline-5- carboxylate (P5C) reductase
        Distance: 0; Region: intron3 (CDS)
      • AT5G14800.2 - [+] show detail - pyrroline-5- carboxylate (P5C) reductase
        Distance: 0; Region: intron3 (CDS)
    Flanking Genome Sequence: 53-4243-1 - 5-prime edge 
    ATCCATGGTG TTGCATTGTG AGTTTCTAGA GCTCAATTTG GTTAAAAAAC 
    ATAAAGGTCT CTCCTTTTTG TTTTTAGGAA TGGTCTGGTC AAGATCGATT 
    CATAAGGGTG ATGCCTAATA CACCTGCCGC TGTTGGTGAG GCAGCTTCAG 
    GTATAGTTTA TCAGAGTGTT CGCATTTGTG ATATTTCTGA ATTTTGTTTG 
    TGATTTCTGT TTCTCTCTGA AGCTGTCAAA TGCTTACATT TGCCAATTAT 
    GTTGTGTGTA TATAGTTATG AGCCTTGGCA CAGGAGCAAC GGAAGAGGAT 
    GGAGCAATTG TTGCTAAGTT GTTTGGCGCG GTGGGGAAGA TATTGAAAGC 
    TGATGAGAAA ATGTTTGATG CTGTCACTGG TCTCAGGTAC AGGAAAACAC 
    TGTAGCTGCT GAAATGCTTG AGTTGTTCAA GTCACTAATC AAGAAAATTT 
    CAAACTTTTT ACAGTGGAAG TGGACCAGCA TACATATTTT TAGCAATTGA 
    AGCT

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 4787099 [Map]; Strand: c
      • AT5G14800.1 - [+] show detail - pyrroline-5- carboxylate (P5C) reductase
        Distance: 0; Region: intron3 (CDS)
      • AT5G14800.2 - [+] show detail - pyrroline-5- carboxylate (P5C) reductase
        Distance: 0; Region: intron3 (CDS)
    Flanking Genome Sequence: 53-4243-1 - 3-prime edge 
    CCATGGATAG TGACAACTTA AATGGCAAAT GTATAGGAGT ATAGATGATG 
    CAAAACCTGT AAATCATTCA ACTTGATTCC AGCTGCAACA GAAACCAGAA 
    TCTTATTCTT TGAAAGCT

AT5G14800.1

Model type
Protein coding
Short Description
pyrroline-5- carboxylate (P5C) reductase
Curator Summary
Delta 1-pyrroline-5-carboxylate reductase, catalyzes the final step in proline biosynthesis from glutamate and ornithine.In situ hybridization indicated that under normal growth conditions, the highest concentration of P5CR transcripts occurs in the cortical parenchyma, phloem, vascular cambium and pith parenchyma in the vicinity of the protoxylem. Single gene in Arabidopsis.
Computational Description
pyrroline-5- carboxylate (P5C) reductase (P5CR); FUNCTIONS IN: pyrroline-5-carboxylate reductase activity; INVOLVED IN: response to salt stress, response to heat, proline biosynthetic process; LOCATED IN: cell wall, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: NADP oxidoreductase, coenzyme F420-dependent (InterPro:IPR004455), NAD(P)-binding domain (InterPro:IPR016040), Delta 1-pyrroline-5-carboxylate reductase (InterPro:IPR000304); Has 7885 Blast hits to 7882 proteins in 2434 species: Archae - 115; Bacteria - 5234; Metazoa - 373; Fungi - 241; Plants - 70; Viruses - 0; Other Eukaryotes - 1852 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G14800.2

Model type
Protein coding
Short Description
pyrroline-5- carboxylate (P5C) reductase
Curator Summary
Delta 1-pyrroline-5-carboxylate reductase, catalyzes the final step in proline biosynthesis from glutamate and ornithine.In situ hybridization indicated that under normal growth conditions, the highest concentration of P5CR transcripts occurs in the cortical parenchyma, phloem, vascular cambium and pith parenchyma in the vicinity of the protoxylem. Single gene in Arabidopsis.
Computational Description
pyrroline-5- carboxylate (P5C) reductase (P5CR); CONTAINS InterPro DOMAIN/s: NADP oxidoreductase, coenzyme F420-dependent (InterPro:IPR004455), NAD(P)-binding domain (InterPro:IPR016040), Delta 1-pyrroline-5-carboxylate reductase (InterPro:IPR000304); Has 7429 Blast hits to 7426 proteins in 2378 species: Archae - 102; Bacteria - 5104; Metazoa - 368; Fungi - 229; Plants - 70; Viruses - 0; Other Eukaryotes - 1556 (source: NCBI BLink).
Link
InterPro Scan - TAIR