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11-6741-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 1579924 [Map]; Strand: c
      • AT4G03560.1 - [+] show detail - two-pore channel 1
        Distance: -132; Region: promoter
    Flanking Genome Sequence: 11-6741-1 - 5-prime edge 
    CCCTCGTCGA TTTCTTGCCA CGTGGCTTTA ATAGCTCAAT TCTGTTTTTT 
    ATTCCCCAAT TCACACTGAT TGTTTCCCAA TTTGTAATAG AAAAGGGAGC 
    AAGAAGTTCA GGATCATCCA AGTAGAAAAG TCAAAACCGA CAAAGTGGTG 
    TTGTGGTCCA ATTATATAAA TTTATTTCAA TTTTGGACCC AGAAATTGAC 
    AAGCAGAAAC CGATAATAGA CGAGAGTAGT GGAATATGGA CAAGTATCTA 
    ATCTGAAACT GATCTCTACT AAGAAGGAGA AGAAAAGATG TGAAATGTGA 
    ACAAAAGCTC TGCGAGATGA CATTAGATAA CAATCTGAAT ATAAACTAAA 
    AATGCTCTAA AGCTCTATAA TGAGAGAAGG AGAACAAATA CACAAAGCCT 
    CACTACACAA ATTGCTGTAC CTTTAGAACA AAAACCATAC AAGAATCAGA 
    GTTCCCTCCA CACACACTGA CAATCAAATA TCTCCCTAAA CCCATCTAAC 
    CGAATCAACC AACTTCCCGA TTCCTCATCC AACCAAACTC TCCAAAAACC 
    TGTTTAAAAT ACAAAGATAA ATCATCAGCA TCTGAGTTTT TCTTTAGACN 
    AAACAAAACT TNGAATT

AT4G03560.1

Model type
Protein coding
Short Description
two-pore channel 1
Curator Summary
Encodes a depolarization-activated Ca(2+) channel. Anti-sense experiments with this gene as well as Sucrose-H(+) symporters and complementation of yeast sucrose uptake mutant cch1 suggest that this protein mediates a voltage-activated Ca(2+ )influx. Mutants lack detectable SV channel activity suggesting TPC1 is essential component of the SV channel. Patch clamp analysis of loss of function mutation indicates TPC1 does not affect Ca2+ signaling in response to abiotic and biotic stress.
Computational Description
two-pore channel 1 (TPC1); FUNCTIONS IN: voltage-gated calcium channel activity, calcium channel activity; INVOLVED IN: regulation of jasmonic acid biosynthetic process, calcium ion transport, calcium-mediated signaling, seed germination, regulation of stomatal movement; LOCATED IN: vacuolar membrane, plasma membrane, vacuole, plant-type vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: EF-HAND 2 (InterPro:IPR018249), Ion transport (InterPro:IPR005821), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR